Hormone Binding and Target Cell Activation in the Testis by K. J. Catt, T. Tsuruhara, C. Mendelson (auth.), Maria L. PDF

By K. J. Catt, T. Tsuruhara, C. Mendelson (auth.), Maria L. Dufau, Anthony R. Means (eds.)

ISBN-10: 1468425951

ISBN-13: 9781468425956

ISBN-10: 1468425978

ISBN-13: 9781468425970

It is curious that study in endocrinology has principally neglected the testis until eventually relatively lately. there have been impor­ tant purposes for this forget; first, tools of analysis have been tricky, and moment, sperinatogenesis was once thought of to be the fear of the urologist or phone biologist yet no longer the endocrinologist. because it is now virtually a moral central that we advance a male contraceptive, and because a bunch of latest recommendations might be dropped at endure on difficulties of testis functionality, study in male reproductive biology has effloresced. actually, it has turn into attainable to undertaking aseries of workshops at the testis, every one facing discrete features of biochemistry, body structure and pathology. it's becoming that this primary Workshop might be on Binding and Activation, seeing that this sector is without doubt one of the frontiers in endocrinol­ ogy. At our current expense of growth it's possible that every of the succeeding workshops will likewise collect leaders in a speedily constructing quarter. The nationwide Institute of kid health and wellbeing and Human improvement has the foremost Federal respon­ sibility in reproductive biology, and has consequently agreed to sponsor this and succeeding workshops. On behalf of the Institute and for these contributors of the Committee who've geared up this assembly, I welcome you. i'm fairly yes that this primary Workshop at the Testis will start up aseries of vital contributions to medical idea in male reproduc­ tive biology. Mortimer B. Lipsett, M. D.

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1 x 10-4 min-1 • At 37° the dissociation rate was significantly higher. COMPUTER ANALYSIS OF hCG BINDING DATA 39 The time course of the binding of hCG to its receptors could be fitted completely. from zero time until arrival at equilibrium. by using as model the second-order chemical kinetic differential equation (Figure 4). As expected. -----------------~ Temperature hCG 24 0 32 picamoles/liter o z o :::> CD o----------------------~--------------------~ 700 1400 TIME (minutes) o Figure 4. Time course of the association of 125I-hCG with its receptors in the rat testis at 24° ; the experiment was continued until equilibrium was reached.

J. Biol. Chem. 248: 5042, 1973. 15. Lee, C. , andRyan, R. , 4609. 1973. 660, 1949. Biochemistry~: 16. Tsuruhara. , Van Hall, E. , Dufau, M. , and Catt, K. : 463, 1972. COMPUTER ANALYSIS OF hCG BINDING DATA 17. Dufau, M. , Charreau, E. , and Catt, K. S. Letters~: 149,1974. 18. Midgley, A. , In International Congress on Gonadotropins, Bangalore, India, 1973 (in press). 19. Ketelslegers, J. Mo, Knott, Go, and Catt, K. Jo, unpublished observations. 45 CHARACTERISTICS OF SOLUBLE GONADOTROPIN RECEPTORS FOR LH AND HCG Maria L o Dufau, E.

M. L •• Catt, K. , and Tsuruhara. , Biochim. Biophys. Acta 252: 574. 1971. 8. Catt. K. J •• Dufau. M. , J. Clin. Endocr. 34: 123. 1972. 9 Bellisario. R •• Carlsen. R. B. and Bahl. O. P •• J. Biol. Chem. 248: 6796. 1973. 10. Carlsen, R. B •• Bahl, O. , and Swaminathan. , J. Biol. Chem. 248: 6810, 1973. 11. Catt, K. , Acta Endocrinol. (Kbh) 63, Suppl. 142: 222, 1969. 12. Dufau, M. L •• Catt, K. , Proc. Natl. Acad. Sei. USA, ~: 2414, 1972. : 13. Scatchard, G •• Ann. N. Y. Acad. Sei. 14. , J. Biol.

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Hormone Binding and Target Cell Activation in the Testis by K. J. Catt, T. Tsuruhara, C. Mendelson (auth.), Maria L. Dufau, Anthony R. Means (eds.)

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