By Aharon Razin, Howard Cedar, Arthur D. Riggs (auth.), Aharon Razin, Howard Cedar, Arthur D. Riggs (eds.)
During the previous few a long time we've witnessed an period of exceptional progress within the box of molecular biology. In 1950 little or no was once identified of the chemical structure of organic platforms, the way during which info was once trans mitted from one organism to a different, or the level to which the chemical foundation of lifestyles is unified. the image this present day is dramatically various. we've got a nearly bewildering number of info detailing many alternative elements of lifestyles on the molecular point. those nice advances have introduced with them a few breath-taking insights into the molecular mechanisms utilized by nature for rep licating, allotting and editing organic details. we now have discovered greatly concerning the chemical and actual nature of the macromolecular nucleic acids and proteins, and the way within which carbohydrates, lipids and smaller molecules interact to supply the molecular surroundings of dwelling sys tems. it'd be acknowledged that those few a long time have changed a close to vacuum of data with a really huge surplus. it really is within the context of this flood of data that this sequence of monographs on molecular biology has been geared up. the assumption is to compile in a single position, among the covers of 1 ebook, a concise evaluate of the nation of the topic in a well-defined box. this can let the reader to get a feeling of historic perspective-what is understood concerning the box today-and an outline of the frontiers of analysis the place our wisdom is expanding steadily.
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Extra resources for DNA Methylation: Biochemistry and Biological Significance
Purified EcoP15 and a crude extract from a res- mod+ deletion mutant of EcoP15 were electrophoresed in the presence of sodium dodecyl sulfate, and the gel was then reacted with the EcoP15 antibody. Although the two subunits were detected in EcoP15, only the smaller one was found in the mutant extract. The same experiment was repeated with EcoPI and a res-mod+ mutant of PI. Two bands were seen when EcoP15 was reacted with the EcoPl5 antibody, but only the smaller subunit was detected in the mutant extract.
Hadi SM, Yuan R: Complementation in vitro by mutant restriction enzymes from Escherichia coli K. J Bioi Chem 1974;249:4580-4586. Type I and Type III Restriction-Modification Enzymes 35 Hadi SM, Bachi B, Shepherd JCW, Yuan R, Ineichen K, Bickle TA: DNA recognition and cleavage by the EcoP15 restriction endonuclease. J Mol Bioi 1979; 134:655-666. Hadi SM, Bachi B, Iida S, Bickle TA: DNA restriction-modification enzymes of phage PI and plasmid p15B: subunit functions and structural homologies. J Mol Bioi 1983; 165:19-34.
Actinomycin D and heparin inhibit both endonuclease and methylase activities. methylated recognition complexes were incubated with either inhibitor before ATP addition, no DNA methylation was detected. When unmodified complexes were used in the same experiment, no inhibition of DNA cleavage took place. Therefore, EeaK bound to hemimethylated sites was susceptible to inhibition by Actinomycin D and heparin, whereas EeaK bound to un methylated sites was resistant. The Effect of ATP on Recognition Complexes The interaction of ATP with the recognition complexes commits each one to its final course of action.
DNA Methylation: Biochemistry and Biological Significance by Aharon Razin, Howard Cedar, Arthur D. Riggs (auth.), Aharon Razin, Howard Cedar, Arthur D. Riggs (eds.)