By R. S. Asquith
This quantity arose initially from the court cases of the editor's scholars, either undergraduate and postgraduate, that there has been no sleek e-book on protein fibers which informed adequate approximately protein technological know-how and chemical tech nologies regarding fibers. most commonly this can be most likely an inexpensive cri de coeur. The undergraduate on a technological path, missing info at the easy medical concepts used to hold out the examine on which his fiber expertise relies, can locate it tricky to procure this knowledge. The natural technology undergraduate usually lacks wisdom of the appliance of those recommendations in protein fiber expertise. The younger graduates, com mencing learn relating to a few element of protein fibers, are drawn from a variety of clinical disciplines, having been proficient as biochemists, chemists, physicists, technologists, and histologists, to call yet a couple of. regularly those new examine employees go through a initial "lost" interval during which they must review their history relating to the large and differing fields of study in protein fiber technology to which they're now uncovered. As time is going on they then both boost a large wisdom masking technological know-how and know-how or stay in a particular a part of their unique self-discipline, with a slim wisdom of its software within the box of the examine measure they're taking.
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Additional resources for Chemistry of natural protein fibers
Protein thiol groups add to 4-vinylquinoline to give derivatives that absorb at 318 nm. (179) Complete hydrolysis of the protein after reaction with the thiol reagent, followed by estimation of the modified half-cystine residues by amino acid analysis, is the basis of the methods in which thioglycollic acid,(68) 4-vinylpyridine,(180) or 2-vinylpyridine(18oa) are used. Close control of the conditions of hydrolysis is required, as the cysteine derivatives tend to be labile. Hydrolysis of the protein before the application of a reaction characteristic of thiols is exemplified by the phosphotungstic acid reduction method(174) associated with the names of Folin and Shinohara.
Separation of Mixtures of Peptides Produced by Chain Cleavage In the fractionation of complex mixtures, an initial subfractionation on as large a scale as possible. even if optimum resolution is not attained, is often a desirable preliminary to the use of techniques of higher resolving power. Column methods, which can be scaled up to deal with substantial loads, are an obvious first choice. 1. , when cyanogen bromide has been used to cleave chains at methionyl residues(215»). High concentrations of urea or formic acid may be employed when the components present have a tendency to aggregate.
Cleavage by Enzymes Acting on Modified Proteins A useful extension of the technique of digestion with enzymes of high specificity is afforded by chemical modification of amino acid side chains. Thus trypsin, which acts at peptide bonds involving arginine or lysine residues, can be restricted to act at only one of these types of residue if the other is chemically modified so that its side chain no longer fulfils the specificity requirements of the enzyme. Further, if the chemical modification can be reversed, the high specificity of trypsin can be utilized again to split the peptides separated from the first digest, after the modifying group has been removed.
Chemistry of natural protein fibers by R. S. Asquith