By Valdur A. Saks, Renée Ventura-Clapier
Most of the knowledge at the useful function, constitution and molecular biology of creatine kinase is scattered in different hundreds of thousands of guides. The editors of this quantity invited all authors who're so much lively at the moment in experimental examine on creatine kinases to summarize their paintings and make the data simply to be had to a much wider clinical viewers, particularly to younger investigators who're getting into this box. because the sensible position of coupled creatine kinases is at once on the topic of the phenomenon of compartmentation and structural association of metabolic networks, we additionally invited specialists within the comparable parts to give a contribution to the quantity. just about all invited authors replied enthusiastically and the result of this collective paintings are offered the following.
For biochemists, biophysicists, physiologists, and clinical examine staff.
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Extra info for Cellular Bioenergetics: Role of Coupled Creatine Kinases
The K+e generally increased during either ischemic or hypoxic insult and returned to baseline upon discontinuation of the insult. However, the magnitude of the changes in K+e are somewhat smaller than those reported by other investigators [129-135]. This may be related to the fact that our measurements were made with surface electrodes placed on the epicardium, whereas measurements by other investigators were made with electrodes introduced into the myocardium. The electrodes used for intramyocardial placement may have damaged some myocytes during their introduction.
Measurement of the specific activity of glucose-6-phosphate lent further support to this hypothesis . Under anaerobic conditions, a detectable decrease in the specific activity of lactate was observed, indicating that unlabelled lactate arising from glycogen was produced. Recent studies of Hardin et al.  provide further support for the separation of glycogenolytic and glycolytic metabolism. In this study, carbon 13 NMR techniques were used to identify the fate of glycogen and glucose carbon.
32. 33. 34. 35. 36. heart in vivo by phosphorus nuclear magnetic resonance. Proc Natl Acad Sci. USA 77: 299-302,1980 Whitman GJR, Chance B, Bode H, Maris J, Haselgrove J, Kelley R, Clark BJ, Harken AH: Diagnosis and therapeutic evaluation of a pediatric case of cardiomyopathy using phosphorus-31 nuclear magnetic resonance spectroscopy. JACC 5: 745-749, 1985 Kantor HL, Briggs RW, Balaban RS: In vivo 31p nuclear magnetic resonance measurements in canine heart using a catheter coil. Circ Res 55: 261-266, 1984 Koretsky AP, Wang S, Murphy-Boesch J, Klein MP, James TL, Weiner MW: 31p NMR spectroscopy of rat organs, in situ, using chronically implanted radiofrequency coils.
Cellular Bioenergetics: Role of Coupled Creatine Kinases by Valdur A. Saks, Renée Ventura-Clapier